This article explores the following questions: What environmental and cultural factors might explain variation in prejudice across cultures? Do these factors explain the intention to vote for nationalist politicians? The authors perform seven studies, focusing on the link between cultural tightness and the rise of prejudice in cultures. They theorize that cultural tightness is positively correlated with the rise of prejudice against people perceived as disrupting the social order. From this theory, they suggest three hypotheses: 1) cultural variation in tightness is related to cultural variation in prejudice, 2) cultural tightness is related to the support for nationalist politicians, and 3) cultural tightness is a link between ecological threats and prejudice. The results support these hypotheses, offering a cultural evolutionary perspective on prejudice.